Our list of good practices in (meta)genome assembly =================================================== Lex Nederbragt (corr) et al. * talk to the bioinformatician(s) before doing anything * QC your reads with fastqc, preqc (khmer?) * try different programs for assembly (not too many, but more than one) * map the reads back to the assembly and use QC/Validation programs * Use orthogonal data for QC/Validation * known genes * CEGMA/Phylosift * RNA-seq data * linkage map data/optical mapping data/fosmids or BAC data * do blobology to figure out what you actually assembled (for any genome/metagenome) * make reads, mapped reads and validation results available upon release of the genome (or before) * make the genome assembly work reproducible